ELISA for IL-6

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Pharmacology
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Company:

Novus Biologicals

Product Name:

IL-6 ELISA Kit

Catalog Number:

NBP2-31051

My project is related to sepsis and some possible inhibitors of sepsis related receptors. We wanted to check how my inhibitors are effective, so as an important proinflammatory cytokine, IL-6, was one of the key players there. For this issue we used the kit from Biocompare, and now we got our results and they look promising.

Experimental Design and Results Summary

Application

Cytokine production

Starting Material

Monocyte cells

Protocol Overview

Incubation of the plate overnight with capture antibody. Then block the plate and add your samples. Afterwards, add the detection antibody and the enzyme. Then add the substrate and the stop solution. Analyze with the plate reader.

Tips

Don't forget to check the concentration of the standards when you prepare them.

Results Summary

Inhibitory effect of some small-molecule designed drugs

Additional Notes

None

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Summary

The Good

Easy to use

The Bad

None

The Bottom Line

I suggested this product to some colleagues.

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