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Purified anti-mouse CD274 (B7-H1, PD-L1)
124302
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This antibody gives good detection of anti-PDL1 in mouse model. Poor cross-reactivity and background.
Immunofluorescence
Glioblastoma tumor - mouse model
Overnight at 4C
Donkey serum
2h RT
None
Confocal
Block your tumor slides - I used 5% donkey serumIncubate O/N at 4C - Ab 1:100 or 1:200wash 3x 10 minincubate 2h RT with you secondary abwash 3x 10 min
It's one of the few anti-mouse PDL1 antibodies that actually works.
Some background present.
Best Anti-mouse PDL1 for IHC that I have tested so far.