Epithelial Progenitor Marker

Department of Urology
UT Southwestern Medical Center
Research Associate

Overall

Quality of Results

Ease-of-Optimization

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Company:

BD Biosciences

Product Name:

CD338 PerCP/Cy5.5

Catalog Number:

561460

Flow cytometry was used to probe epithelial dynamics and the immune infiltrates in human prostate tissues. Epithelia was investigated with CD45-CD325+ cells. Leukocytes were looked at using CD326-CD45+. Sub-populations (such as epithelial stem cells or individual leukocyte populations like T-Cells) were gated with appropriate markers. This antibody stains rare epithelial progenitors.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Primary human prostate cells

Primary Incubation

Recommended for 20-30mins

Blocking Agent

FcX

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

Flow Cytometer

Results Summary

CD45- CD326+ CD338+ = Epithelial Progenitors

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

A large number of positive cells co-stain with other progenitor markers

The Bad

Not clear populaiton

The Bottom Line

Rare population, good Ab

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