SLC18A2 Staining in Human Pancreas Tissue

May 09, 2016

Molecular Physiology and Biophysics
Vanderbilt University Medical Center
Postdoctoral Research Fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

MyBioSource

Product Name:

Anti-SLC18A2 antibody

Catalog Number:

MBS833162

We are optimizing staining conditions of SLC18A2 in human cryoembedded tissue. This antibody works best at 1:100 dilution.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Human pancreas tissue

Primary Incubation

1:100, overnight, 4 deg Celcius

Blocking Agent

5% Normal Donkey Serum in 1%BSA/PBS

Secondary Incubation

1:1000, Donkey anti-mouse Cy3

Tertiary Incubation

NA

Detection

Immunofluorescence

Results Summary

SLC18A2 stained human islet beta cells, as expected. It was easily detected at 1:100 dilution with little background in other tissues.

Additional Notes

None

Related Categories

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Summary

The Good

Excellent detection at 1:100

The Bad

Need 1:100 dilution to achieve optimal staining, which could be costly in the long run

The Bottom Line

SLC18A2 stained islet beta cells in human tissue, as expected. Only downfall is the high concentration used (1:100) and the price of the antibody. (>$500/vial)

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