NFATc1 Antibody on Macrophage and Osteoclast Cells

Results Summary

We used WT or KO murine bone marrow macrophages deficient in their ability to differentiate into osteoclasts and either retrovirally overexpressed an empty vector or NFATc1 construct to rescue the KO's differentiation defect. As observed, in bone marrow macrophages, NFATc1 expression in empty vector cells are low, as expected. With NFATc1 overexpression in macrophages, there is increased expression of this protein. As the WT cells differentiate into mature osteoclasts, in empty-vector samples, we see native upregulation of NFATc1, and blunted upregulated in the KOs. However NFATc1 overexpression is strongly exhibited in both WT and KO osteoclasts. 10 ug of protein was lysed in RIPA buffer per lane.