BioLegend
FITC Thy1.1
202503
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The aim of the current project to investigate how memory CD8 T cells are formed after viral or bacterial infections. We use the lymphocytic choriomeningitis virus (LCMV) and listeria infection mouse models to address this question. To track the in vivo differentiation of CD8 T cells, a small amount of TCR transgenic P14 cells (specifially recognize the GP33-41 epitope on LCMV and listeria-gp33 pathogens) are transferred into host mice, which are then infected. Those P14 cells, which express the congenic markers (such as CD90.1, or Thy1-1) were subject to phenotypic and functional analysis afterwards.
Flow Cytometry
Mouse cells
1:100 dilution; on ice for 30 minutes.
Homemade 2.4G2 blocker.
No secondary incubation
No tertiary incubation
FACS
In this experiment, the P14 donor mice were genotyped. The antibody was used to determine the congenic marker expression of the donor mice. Biolegend antibodies have a good reputation, so we choose this antibody for our experiment.
None
This antibody has a strong signal and it is very easy to use.
As shown in the attached figure, the donor cells have a clear CD90.1 positive population. Thus, we will use CD90.1 to track these donor cells in vivo.