Anti-SMAGP Antibody for Western-Blot

Tumor Biology
German Cancer Research Center
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Atlas Antibodies

Product Name:

Anti-SMAGP antibody produced in rabbit

Catalog Number:

HPA039711 Sigma

The main goal of our research is to identify new genes that play critical roles in cancer formation and development. To characterize the candidate genes and examine their cellular functions, we perform several cell-based assays. Having a specific antibody that is able to detect the target protein is a fundamental point in our experimental model.

Experimental Design and Results Summary

Applications

Western Blot

Sample

MCF7, Caco-2 cell lysates

Primary Incubation

1:8000 diluted in 1% BSA, incubation overnight

Blocking Agent

5% milk powder solution

Secondary Incubation

HRP coupled secondary antibody diluted 1:5000 in 1% BSA for 30 min at room temperature

Tertiary Incubation

NA

Detection

ECL detection

Results Summary

Detection of specific bands for SMAGP in the examined cell lysates

Additional Notes

None

Related Categories

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Summary

The Good

Specific detection of SMAGP

The Bad

None

The Bottom Line

Good antibody for the Western-Blot of SMAGP

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