Anti-MAPRE2 Antibody for Immunofluorescence

May 03, 2016

Tumor Biology
German Cancer Research Center
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Atlas Antibodies

Product Name:

Anti-MAPRE2 antibody produced in rabbit

Catalog Number:

HPA016739 Sigma

The main goal of our research is to identify new genes that play critical roles in cancer formation and development. To characterize the candidate genes and examine their cellular functions, we perform several cell-based assays. Having a specific antibody that is able to detect the target protein is a fundamental point in our experimental model.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Caco-2 cells

Primary Incubation

1:35 diluted in 1xPBS, incubation for 1h at room temperature

Blocking Agent

NA

Secondary Incubation

Cy3-coupled secondary antibody, 30 min incubation at room temperature

Tertiary Incubation

NA

Detection

Fluorescence microscope

Results Summary

Cytoplasmic staining for MAPRE2 in cells

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Detection of cytoplasmic MAPRE2

The Bad

Low dilution factor

The Bottom Line

Good antibody for the detection of cytoplasmic MAPRE2 in cells

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