Sigma Atlas Antibodies
Anti-NELFB
HPA020259
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Browse Similar Products: NELFB Immunofluorescence Antibodies
The main goal of our research is to identify new genes that play critical roles in cancer formation and development. To characterize the candidate genes and examine their cellular functions, we perform several cell-based assays. Having a specific antibody that is able to detect the target protein is a fundamental point in our experimental model.
Immunofluorescence
Caco-2 cells
1:20 diluted in 1xPBS, incubation for 1h at room temperature
No blocking
Cy3-coupled secondary antibody, 30 min incubation at room temperature
NA
Fluorescence microscopy
Specific nuclear staining in Caco-2 colon carcinoma cells
None
Specific staining
Too low dilution
Good antibody to visualize the cellular localization of the NELFB protein