Agilent Technologies
SURE electrocompetent cells
200227
We need to generate lentiviral vector for our expression studies on the HSC cells. We had problems with lentiviral vector amplification (unstable and more unwanted recombination) in the large culture of bacteria . So we choose these cells because this strain suppressed unwanted recombination.
Faithfull amplification of large constructs-lenti vector
Lenti plasmid/ ligation mixture
We electroporated 2ul ligation mixture in to 40ul of SURE competent cells @1700V, 200 Ohms with single pulse. We followed the other steps as recommended in the Manual.
14hr- optimum yield with less unwanted recombination
We do observe unwanted recombination with our 13 kb lenti viral vector however its comparatively lower compared to other vendor Electro competent cells.
None
Less unwanted recombination
Much less yield
Better for faithfull amplication of lenti vector in a large volume of bacterial culture