Quick Clean and Concentration of Low to High Molecular Size DNA

The Jackson Laboratory
Cancer biology
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Company:

Zymo Research

Product Name:

Oligo Clean and Concentrator

Catalog Number:

D4060

We use a lot of customized oligos (range from 45- 150bp) that require a lot of process such as Klenow end repair and restriction digestion before putting into the plasmid vector. Since these oligos are smaller in size, we used this product to purify the enzymatic reaction with less loss of oligos.

Experimental Design and Results Summary

Application

Clean and concentrating oligos from enzymatic reactions

Starting Material

Double or single strand DNA/oligo

Protocol Overview

The binding buffer, 100% ethanol, sample (DNA) was added 2:8:1 ratio and passed through the column. As recommended per manual washed with 750ul of Wash buffer. The Oligos/plasmid eluted atleast with 15ul of nuclease free water.

Tips

Maintain the buffer ratio to DNA

Results Summary

We used to recover almost 80%-90% oligos from the enzymatic reactions.

Additional Notes

None

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Summary

The Good

Fast and efficient

The Bad

None

The Bottom Line

Best kit to reduce loss and purifiy the DNA during various downstream steps.

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