Good Separation for CD45 Population

April 26, 2016

Pharmaceutical Technology
University of Basque Country
Researcher

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

Miltenyi

Product Name:

CD45-PE-Vio770, mouse (clone: 30F11)

Catalog Number:

130-105-505

This is a good antibody with a nice separation of CD45 positive cells in thymus tissue.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Thymus

Primary Incubation

1:100

Blocking Agent

Blocking reagent from Miltenyi

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

Flow cytometry in PEVio770 or PECy7 channel

Results Summary

Really good separation as it can see on the figure. We selected CD45 positive cells from a single suspensión of thymus and positive cells were easy to detect and quantify.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Good separation

The Bad

None

The Bottom Line

Really happy with this product and I recommend for use.

Share your experience with other scientists. Write a Review! »

Join the discussion