Very Good CD56 Antibody

May 02, 2016

Neurosurgery
Havard medical school
Postdoc

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
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Company:

BD Biosciences

Product Name:

V450 Mouse Anti-Human CD56 Clone B159 (RUO)

Catalog Number:

560361

I am characterizing PBMCs after various treatments and their activation status. For this antibody I would recommend to use a second antibody to better characterize CD56+ from other cells.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

PBMCs

Primary Incubation

30min, RT, in the dark

Blocking Agent

NA

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

V450 Chanel

Results Summary

Good CD56 antibody. I would use a second marker for the detection of NK-cells

Additional Notes

None

Related Categories

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Summary

The Good

Easy to use and optimize

The Bad

Better use a second marker

The Bottom Line

Good human CD56 antibody if used with a second marker for NK cells

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