Okay Human CD8 Antibody

Neurosurgery
Havard medical school
Postdoc

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

BD Biosciences

Product Name:

V500 Mouse Anti-Human CD8 Clone SK1 (RUO)

Catalog Number:

561617

I am characterizing PBMCs after various treatments and their activation status.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

PBMCs

Primary Incubation

30min, RT, in the dark

Blocking Agent

NA

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

V500 Chanel

Results Summary

The antibody is good for detection of CD8+ PBMCs, however you can only use a 1:10 dilution, otherwise the signal gets very weak.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Good antibody for CD8 in combination with CD4

The Bad

Needs to be in a high concentration

The Bottom Line

This antibody is ok for CD8 detection in PBMCs. Signal get weak if you use it in a lower dilution then 1:10

Share your experience with other scientists. Write a Review! »

Join the discussion