So Bright!

Department of Hematology/Oncology
MSSM
Associate Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

BD Biosciences

Product Name:

CD45 BV510

Catalog Number:

563204

CD34 HSCs were isolated from cord blood samples and differentiated into myeloid cell types using a specific media and cytokine mix.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Differentiated myeloid cells from CD34 (cord blood)

Primary Incubation

1:500, 30 minutes, 4deg

Blocking Agent

NA

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

Flow cytometry (LSR Fortessa)

Results Summary

CD45 BV510 was used to identify the hematopoietic populations in the differentiated cultures. CD45 was easily identified using BD's CD45 BV510 with excellent specificity and close to no background.

Additional Notes

None

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Summary

The Good

Bright and specific!

The Bad

Nothing

The Bottom Line

Awesome CD45 antibody

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