Synaptic Systems
m6A (N6-methyladenosine) Polyclonal rabbit antibody
202 003
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Browse Similar Products: m6A Immunoprecipitation Antibodies
Synaptic systems m6A antibody is one of the best antibodies to carry out m6A-related studies.
Immunoprecipitation
mRNA
4 degrees 2 hours
NA
qPCR and high throughput sequencing
For qPCR, intact mRNA was used for m6A Ab IP. Enriched mRNA was tested by qPCR using primers for m6A-modified genes. The enrichment fold is around 20, not ideal but reasonable. For high-throughput sequencing, mRNA was fragmented before subjected to m6A Ab IP. Enriched mRNA fragments containing m6A is used to construct mRNA-seq libraries for high-throughput sequencing. The detection sensitivity toward m6A-modified gene transcripts is excellent. Metagene shows good transcriptome-wide enrichment. Ideal for m6A-seq in various organisms.
None
Good enrichment towards fragmented mRNA. Ideal for sequencing sample preparation.
Mediocre enrichment towards intact mRNA, not ideal for qPCR-based detection.
One of the best commercial-available m6A antibodies.