Surface Expression of DNGR1

April 21, 2016

Immunology
University of Rochester
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

BD Biosciences

Product Name:

DNGRI anti-mouse alexa fluor 647

Catalog Number:

564272

This antibody was used as a preliminary screen to assess DNGR1 expression on naïve DCs in BALB/c.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Lymph node from BALB/c

Primary Incubation

30min on ice. 0.5ul per 100ul test.

Blocking Agent

Fc Block

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

Fortessa

Results Summary

A small population can be distinguished to express low levels compared to isotype.

Additional Notes

None

Related Categories

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Summary

The Good

Ease of use

The Bad

Could be brighter

The Bottom Line

OK antibody to stain cells. Expression is dull.

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