DC SIGN Expression on Mouse DC

April 18, 2016

Immunology
University of Rochester
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

eBioscience

Product Name:

DC SIGN APC anti-mouse

Catalog Number:

17-2092

I needed to use this antibody to assess surface expression of DC SIGN on mouse DCs.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse lymph node cells BALB/c

Primary Incubation

30 min on ice. 0.6ul in 100ul test.

Blocking Agent

Fc Block

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

Fortessa

Results Summary

DC sign expression is very dull on mouse DCs.

Additional Notes

None

Related Categories

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Summary

The Good

Ease of use

The Bad

Not bright enough

The Bottom Line

Expression is detectable using this antibody, however it is dull.

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