CD207 Staining on Mouse DCs in Lymph Node

April 15, 2016

Immunology
University of Rochester
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

BD Biosciences

Product Name:

CD207 AlexaFluor647 anti-mouse

Catalog Number:

564937

This antibody was used to assess surface CD207 staining on naïve DCs in BALB/c

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse lymph node cells

Primary Incubation

30min on ice. 0.5ul in 100ul test.

Blocking Agent

Fc Block 1:100 5min.

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

Fortessa

Results Summary

CD207 expression is very low on naïve DCs in lymph node compared to isotype.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Ease of use

The Bad

Not bright enough

The Bottom Line

Detectable expression, but dull.

Share your experience with other scientists. Write a Review! »

Join the discussion