Heat-Shock Protein 90 Beta

April 20, 2016

Biology
Penn State University
Technician

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
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Company:

Bosterbio

Product Name:

Anti-GRP94

Catalog Number:

PA1340

This is an excellent antibody to endoplasmin in HC11 cells.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Cell lysate

Primary Incubation

1:2000 in TBST/5% BSA overnight 4C w/rocking

Blocking Agent

5% BSA

Secondary Incubation

Anti-rabbit hrp

Tertiary Incubation

NA

Detection

Enhanced chemiluminescence

Results Summary

Clean, reliable detection with very little if any background. 

Additional Notes

None

Related Categories

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Summary

The Good

Reliable and robust

The Bad

No bad

The Bottom Line

Great antibody for Westerns. Could probably be optimized for 1 hour primary incubation.

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