Stbl3 Competent Cells

University of Miami
Cell Biology
Graduate Student

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Quality of Results

Ease-of-Optimization

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Company:

Invitrogen

Product Name:

One Shot Stbl3 Competent Cells

Catalog Number:

C7373-03

These competent cells are used for cloning proviral vectors. The competent cells are excellent for growing viral vectors with LTRs because they are RecA deficient.

Experimental Design and Results Summary

Application

Molecular Cloning

Starting Material

None

Protocol Overview

Mix DNA with cells. Incubate for 30 min. on ice. Heat shock the cells and then chill on ice. Add SOC media for 1 hour and then plate onto bacterial plates.

Tips

Gently mix the cells with DNA

Results Summary

Used for transformation of proviral vectors and have not had issues with recombination.

Additional Notes

None

Image Gallery

Summary

The Good

No recombination

The Bad

Price

The Bottom Line

These are good competent cells for vectors containing LTRs.

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