Good Way to Isolate Neutrophils From Lungs

Results Summary

Between 60 - 95% purity. My samples were from highly infected, inflamed, and damaged murine lungs (Mtb infection) and contain a ton of different inflammatory cells and dying cells, which undoubtedly leads to contamination / problems. There is a clear trend of "contaminating" cells in my preps. The sample (left) that is 62% pure is a wildtype mouse that doesn't have much inflammation or neutrophils in the lungs. The middle sample is a mouse that develops slightly more inflammation and neutrophils in the lungs (82% pure). Finally, the right mouse is a strain that develops severe neutrophillic infiltrate, which eventually takes over the lung space and kills the mouse during infection (97% pure). So, I think these differences result from a standard amount of "contamination" and the higher purity prep (right) is simply a result of the fact that the vast majority of the cells in that mouse are neutrophils, so while some may be non-specifically isolated, it just happens to be what I am interested in. This trend is consistent over 5 strains tested and over 30 samples prepped. But, given the severe inflammatory response and high percentage of dying cells present, this product meets all reasonable expectations for a column based purification. As I noted, it would probably be better to do a dead cell removal kit first, but I haven't tried that yet.