Great Antibody to Detect ELISAs

Department of Pediatrics
University of California, San Diego
Postdoctoral Fellow

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

BD Pharmigen

Product Name:

PE-Cy7 Rat Anti Mouse Ly6G

Catalog Number:

560601

This product was used for flow cytometry, fixable. There have been no dye breakdown problems or compensation issues.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Murine bone marrow, blood and lung

Primary Incubation

1:200 at 4C for 20 minutes

Blocking Agent

FcBlock in PBS or FACS buffer

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

PE-Cy7 (conjugated) and flow cytometry

Results Summary

Great and reliable. No problems with dye breakdown or loss of brightness. Fixed samples in 4% PFA for 20 minutes (for biosafety reasons) and haven't noticed any change or problems with the staining. We have had this antibody for several years, and the last aliquot worked just as well as the first. No major problems with compensation either.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Works consistently

The Bad

None

The Bottom Line

Great for flow cytometry. Works as expected.

Share your experience with other scientists. Write a Review! »

Join the discussion