Abcam
Anti-KIF15
AB90735
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The purpose of this study was evaluation of KIF15 expression across a number of cell lines. We purchased 6-7 antibodies targeting KIF15 to optimize for use in Western blotting, immunofluorescence, etc. This antibody was chosen for convenience (order a lot from Abcam) and price.
Western Blot
Cancer cell line lysates
1:1000 in 5% milk in TBS-T, overnight at 4 degrees C, on a rocker
5% milk in TBS-T
1:2500 - 1:5000, anti-RB in 5% milk in TBS-T, 30 minutes at room temp, on a rocker
NA
HRP Chemiluminescence
Bands were observed in some lines, however, the bands were at 40 kDa and the size of KIF15 is 160 kDa. Use of other antibodies reveals bands of varying intensity in each cell line at 160 kDa, suggesting that this antibody recognizes a protein that is not KIF15.
None
Nothing
Doesn't recognize correct protein
Don't purchase this antibody.