CD3 Receptor Antibody

Internal Medicine
University of California Medical Center
Post doctoral Fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

CD3-AF 700

Catalog Number:

100215

Mouse peripheral lymph node cells were used to stain for CD3 receptor in combination with other cell surface markers. Since CD3 was to be checked on a specific class of cells with stronger color, AF-700 was chosen.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse PLN

Primary Incubation

CD3 AF-700 1:500

Blocking Agent

CD16/32

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

On BD Accuri

Results Summary

Specific staining was obtained for CD3 population. However signal may be increased with voltages.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Specific

The Bad

Needs voltage manipulation a little bit

The Bottom Line

Simple and good antibody for staining with strong colors.

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