Anti Actin Antibody for Western Blot

April 08, 2016

MOLB
University of Wyoming
PhD student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Abgent

Product Name:

ACTA1 antibody

Catalog Number:

AM1965b

Browse Similar Products: Actin Western Blot Antibodies

Anti-actin antibody is used as a loading control of whole cell lysate for Western blot.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Whole cell lysate of radial growth primary melanoma cells WM35

Primary Incubation

1:500, overnight, 4C

Blocking Agent

Odyssey Blocking buffer (PBS)

Secondary Incubation

IRDye anti mouse antibody, 1:20000, 1h, room temperature

Tertiary Incubation

NA

Detection

LI-COR

Results Summary

Anti-actin antibody is used as a loading control. It works great for whole cells lysate for Western blot

Additional Notes

None

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Summary

The Good

Great antibody, no unspecific binding

The Bad

None

The Bottom Line

We tried several anti actin antibodies and this one works the best for Western blot.

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