Percp Anti-CD90-1 Antibody

Immunobiology
Yale University
Postdoc fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

Percp anti-CD90-1

Catalog Number:

202512

The aim of the current project is to investigate how memory CD8 T cells are formed after viral or bacterial infections. We use the lymphocytic choriomeningitis virus (LCMV) and listeria infection mouse models to address this question. To track the in vivo differentiation of CD8 T cells, a small amount of TCR transgenic P14 cells (specifially recognize the GP33-41 epitope on LCMV and listeria-gp33 pathogens) are transferred into host mice, which are then infected. Those P14 cells, which express the congenic markers (such as CD90.1, or Thy1-1) were subject to phenotypic and functional analysis afterwards. In this experiment, the P14 donor mice were genotyped. The antibody was used to determine the congenic marker expression of the donor mice. BioLegend antibodies have a good reputation, so we chose this antibody for our experiment.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse cells

Primary Incubation

1:100 dilution; on ice for 30 minutes.

Blocking Agent

Homemade 2.4G2 blocker.

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

FACS

Results Summary

As shown in the attached figure, the donor cells have a clear CD90.1 positive population. Thus, we will use CD90.1 to track these donor cells in vivo.

Additional Notes

None

Related Categories

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Summary

The Good

This antibody gives a very strong and clear signal with low background noise.

The Bad

None

The Bottom Line

This antibody has a strong signal and it is very easy to use.

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