BioLegend
Purified anti-TCR Va 2
127802
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The aim of the current project is to investigate how memory CD8 T cells are formed after viral or bacterial infections. We use the lymphocytic choriomeningitis virus (LCMV) and listeria infection mouse models to address this question. To track the in vivo differentiation of CD8 T cells, a small amount of TCR transgenic P14 cells (specifially recognizes the GP33-41 epitope on LCMV and listeria-gp33 pathogens) are transferred into host mice, which are then infected. Those P14 cells were subject to phenotypic and functional analysis afterwards. In this experiment, the P14 donor mice were genotyped. The antibody was used to determine if the candidate donor mice bear the TCR transgene P14. BioLegend antibodies have a good reputation, so we chose this antibody for our experiment.
Flow Cytometry
Mouse cells
1:100 dilution; on ice for 30 minutes.
Homemade 2.4G2 blocker.
1:500 dilution; on ice for 30 minutes with R-PE conjugated secondary antibody
NA
FACS
As shown in the attached figure, the cells tested have a very high mean fluorescent intensity (MFI), suggesting the cells are P14 transgenic cells.
None
This antibody gives a very strong and clear signal with low background noise.
This antibody has a strong signal and it is very easy to use.