Anti-TPP1 for Mouse Samples

Environ. Med.
University of Rochester
Postdoc

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Quality of Results

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Company:

Abcam

Product Name:

Anti-TPP1 antibody [EPR16537] (ab195234)

Catalog Number:

ab195234

We tested the levels of TPP1 in mouse lung to determine the role of TPP1 during oxidative stress.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Whole lung homogenate

Primary Incubation

1:1000 in 5% milk at 4 degrees overnight

Blocking Agent

5% Milk

Secondary Incubation

1:10,000 Goat Anti-Rabbit IgG, (H+L), HRP conjugate

Tertiary Incubation

NA

Detection

ECL

Results Summary

We were able to detect the basal levels of TPP1 in mouse lung homogenates.

Additional Notes

Works with some additional bands along with TPP1 target band

Related Categories

Image Gallery

Summary

The Good

Works in mouse lung samples

The Bad

Some nonspecific bands observed

The Bottom Line

Works in lung homogenates

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