Invitrogen
Electro Max Stbl4 Cells
11635018
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Stbl4 electro competent cells have high transformation efficiency which is useful for those who want to make a complex library directly from ligation mixture and culturing transformation mixture instead of plating them to a single colony.
To make a highly complex library preparation
Lentiviral plasmid
The ligation (concentrated from several ligation mixtures) was electroporated (2ul) in up to 20ul of Stabl4 cells @ 1200Kv, 25uF transformations were grown at 37C for 1hr. After that, the transformation was transferred to a 100ml culture overnight at 37C. The plasmid DNA was isolated by Maxi prep. The lenti library prepared from stabl4 was shown to be highly diverse compared to other competent cells.
Direct electroporation of ligation needs 90 min recovery time.
We made a highly complex library with diversity about 100000 different clones from plasmid size 15kb.
None
Good for complex library making
Good electrocompetent cells to complex library making.
Product Name: ElectroMAX Stbl4 Competent CellsSupplier Page