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Alexa Fluor700 anti-human CD14 Antibody
325614
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Browse Similar Products: CD14 Flow Cytometry Antibodies
This antibody was used in a pediatric study involving healthy and asthmatic patients. Multiple phenotyping panels were used to screen these patients for any changes in cell populations. Monocytes are part of one of these panels. This antibody works well to separate the classical monocytes CD14+ CD16- from intermediate monocytes CD14+ CD16+.
Flow Cytometry
Human PBMC/monocytes from whole blood
2/100 dilution in final volume of 50ul (PBS+ 2%FBS) for 15min at RT at dark.
FcR block (Miltenyi) for 3min at RT
NA
LSRII (Becton Dickinson)
Nice separation of CD14+ monocytes from whole blood and easy separation of classical monocytes CD14+ from intermediate monocytes by adding CD16 antibody (BV570, cat#302035, Biolegend).
Stock concentration of 0.5Mg/ml was diluted to a working concentration of 20ug/ml for a final concentration of 1ug/ml during staining.
Nice separation
None
Nice separation of CD14+ monocytes from whole blood.