Thermo Fisher Scientific
Qubit® dsDNA HS Assay Kit
Q32854
Info: View Product View Product Specs
I used Qubit dsDNA HS assay kit to quantify genomic DNA from bacterial and as well human cell lines. The kit was very specific to dsDNA and sensitivity was spot on which can detect even up 2 ng per microliter.
Quantification of genomic DNA for cloning and sequencing
Genomic DNA from human intestinal epithelial cells
Genomic DNA was used diluted into 1:5 ration and this diluted genomic DNA was used at 2 microliter with 198 microliter of Qubit dsDNA HS buffer prepared with qubit reagent (1:200). Then fluorescence was recorded in Qubit 2.0 Fluorimeter and quantification was extrapolated from standard curve analysis.
Dilute your genomic DNA if the concentration is very high
Got good yield from my extraction procedure and quantities were 50 to 80 ng/ microliter
None
Very easy to perform and takes less time to complete the protocol.
We prefer this kit as we do lot of sequencing projects.
Product Name: Qubit dsDNA HS Assay KitSupplier Page