Anti-Mouse/Rat IL-17A

March 25, 2016

Medicine
Harvard Medical School
Post-doctoral fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

eBioscience

Product Name:

Anti-Mouse/Rat IL-17A PE

Catalog Number:

12-7177-81

I studied immune response in mice against mycobacterium tuberculosis H37Rv and clearance of the pathogen from mice's systems using anti-mycobacterial ESAT6 binding peptides.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Murine Lymphocytes

Primary Incubation

1 hour on ice with primary antibodies

Blocking Agent

Blocking with 2% BSA and Fc block (1:100)

Secondary Incubation

1:50 dilution on ice with intracellular staining antibodies

Tertiary Incubation

NA

Detection

FACS

Results Summary

IL17a expression was detected on CD8+ lymphocytes

Additional Notes

None

Related Categories

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Summary

The Good

Good flow cytometry antibody for IL-17 intracellular staining

The Bad

None

The Bottom Line

Detected IL-17 expression on lung lymphocytes on CD8+ population in response to mycobacterial antigens.

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