Review for Phenomenex Biosep-S4000 HPLC Column

March 28, 2016

University of Wisconsin
Radiology
Postdoc fellow

Overall

Performance

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

Phenomenex

Product Name:

BioSep-SEC-S4000, 300*7.8 mm

Catalog Number:

00H-2147-K0

An SEC column (Phenomenex®BioSec-SEC-4000, 300*7.8 mm) was used for the purification of DNA tetrahedral nanoparticles, and all chromatograms were collected at 260 nm. A typical SEC mobile phase was 450 mM NaCl, 25 mM Tris base (pH 7.3). 10~500 μL of as-prepared TDNs was loaded and eluted at a flow rate of 0.6 mL/min with isocratic elution. The retention time of TDNs was commonly in the range of 15~16 min. Reference: ACS Appl Mater Interfaces. 2015 Jun 24;7(24):13174-9. doi: 10.1021/am505592e.

Experimental Design and Results Summary

Application

Size exclusion chromotography

Starting Material

None

Protocol Overview

This column was used for the purification of TDNs, and a typical SEC mobile phase was 450 mM NaCl, 25 mM Tris base (pH 7.3). 10~500 μL of samples was loaded and eluted at a flow rate of 0.6 mL/min with isocratic elution. The retention time of DNA nanostructures was commonly in the range of 15~16 min.

Tips

Easy and efficient to use

Results Summary

Could provide rapid and efficient purification for DNA nanostructures or DNA-peptide conjugates.

Features Summary

Really easy to use.

Additional Notes

None

Image Gallery

Summary

The Good

Easy to use, could provide good purification results.

The Bad

Everything works fine for purification of DNA nanostructures or DNA-peptide conjugates.

The Bottom Line

The column provides a very good resolution within a relatively short period, and it is really easy to use.

Join the discussion