UltraComp: One Compensation Bead for All Isotypes

March 17, 2016

University of Rochester
MBI
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

eBioscience

Product Name:

UltraComp eBeads

Catalog Number:

01-2222-42

These beads were used to determine spectral spillover for compensation.

Experimental Design and Results Summary

Application

Flow Cytometry

Starting Material

UltraComp Compensation Beads

Protocol Overview

Incubation 1:200 of various antibody isotypes. Detection with LSRII Flow Cytometer.

Tips

None

Results Summary

UltraComp beads stained brightly with multiple isotypes.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Stains with multiple isotypes

The Bad

None

The Bottom Line

Works with multiple antibody isotypes allowing the lab to only stock one compensation bead vial.

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