BD Biosciences
PE Hamster Anti-Mouse CD80
553769
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Browse Similar Products: CD80 Flow Cytometry Antibodies
CD80 was used to identify activated murine myeloid cells in spleen. Murine splenocytes were incubated with LPS over night to induce activation and then stained with CD11c and MHCII to identify dendritic cells. Anti-CD80 was then used to measure activation of these cells. PE was chosen as fluorochrome due to its brightness.
Flow Cytometry
Murine splenocytes
1:400, 15min, 4C
Blocking Buffer containing sera (rat, rabbit, human, goat)
NA
Flow cytometry (BD) LSRFortessa
The antibody is very bright and allows for easy dsicrimination between the positive and negative populations. Due to its brightness it can also be used in relatively low concentrations.
None
Bright, easy to use
No complaints
Easy to use, bright antibody