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PE/Cy7 anti-mouse CD40 Antibody
124622
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Browse Similar Products: CD40 Flow Cytometry Antibodies
CD40 was stained on murine splenocytes to identify activated myeloid cells. Splenocytes were incubated with LPS overnight to induce activation of myeloid cells and then stained with CD11c and MHCII to identify dendritic cells. Anti-CD40 was used to measure activation of these cells. We chose PE-Cy7 due to its brightness to allow good discrimination between positive and negative cells.
Flow Cytometry
Murine splenocytes
1:400, 15min, 4C
Blocking buffer containing sera (rat, rabbit, human, goat)
NA
Flow cytometry (BD) LSRFortessa
The antibody allows for easy identification of positive cells. Due to its brightness it can be used in relatively low concentrations.
None
Bright fluorophore
Discrimination between positive and negative populations could be a little better
Easy to use, good staining quality, bright fluorophore