Santa Cruz Biotechnology
PCNA (F-2)
sc-25280
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The goal of this experiment was to measure PCNA via western blot to assess cell proliferation in an H4IIE rat hepatoma cell line. We selected this product based on a recent paper that used it to measure proliferation.
Western Blot
Total cell lysate of H4IIE rat hepatoma cells
1:200 over night at 4 degrees C.
1:3 Odyssey Block:TBS for 1 hour at room temperature
1:3 Odyssey Block:TBST (0.1% Tween and 0.02% SDS) for 1 hour at room temperature
NA
Odyssey Infrared Imager
Successful detection of PCNA. Would suggest a 1:500-1:1000 dilution for best results.
None
Strong signal
PCNA works well when detecting via western blot.