Anti-Human/Mouse CD44

March 21, 2016

Medicine
Harvard Medical School
Post-doctoral fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

eBioscience

Product Name:

Anti-Human/Mouse CD44 FITC

Catalog Number:

11-0441-82

I studied immune response in mice against mycobacterium tuberculosis H37Rv and clearance of the pathogen from mice's systems using anti-mycobacterial ESAT6 binding peptides.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Murine Lymphocytes

Primary Incubation

1:100 dilution for 1 hour on ice

Blocking Agent

Blocking with 2% BSA and Fc block (1:100)

Secondary Incubation

None

Tertiary Incubation

None

Detection

FACS

Results Summary

Detected CD44 expression in lung lymphocytes on CD4+ population in response to mycobacterial antigens.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Efficient CD44 expression detection

The Bad

None

The Bottom Line

Good flow cytometry antibody, gives nice signal

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