Good Anti-Rabbit HRP-Linked Secondary Antibody

Medicine
Harvard Medical School
Post-doctoral fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technology

Product Name:

Anti-rabbit IgG, HRP-linked Antibody

Catalog Number:

7074

I studied immune response in mice against mycobacterium tuberculosis H37Rv and clearance of the pathogen from mice's systems using anti-mycobacterial ESAT6 binding peptides.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Whole cell lysates

Primary Incubation

1:1000 at 4 degrees for overnight incubation

Blocking Agent

5% skimmed milk in 1X PBS

Secondary Incubation

1:2000 at room temperature for 2 hour

Tertiary Incubation

NA

Detection

Western Blot

Results Summary

Antibody has efficient binding to rabbit raised primary antibodies.

Additional Notes

None

Image Gallery

Summary

The Good

Less backgound

The Bad

None

The Bottom Line

Good secondary antibody for western blotting, gives less background.

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