eBioscience
Anti-Mouse CD8b APC
17-0083-81
Info: View Product View Product Specs
Browse Similar Products: CD8b Flow Cytometry Antibodies
The goal of this experiment was to clearly distinguish CD8+ T cells in the peripheral blood of female C57Bl/6 mice for subsequent phenotyping. I selected this product because CD8b, not CD8a, is specific in expression to T cells.
Flow Cytometry
Peripheral blood leukocytes
Stained cells in 100 uL cold PBS containing Live/Dead and TruStain Fc block (2 ug/mL) on ice for 15 minutes.
TruStain Fc block (2 ug/mL)
Added 100 uL cold PBS containing isotype control (4 ug/mL) or CD8b APC (4 ug/mL) and incubated on ice for 15 minutes.
NA
FACS Canto
CD8b+ T cells in the peripheral blood leukocytes were completely distinguishable from CD8- cells.
None
Inexpensive, clearly distinguishes CD8b+ T cells
A solid antibody for phenotyping T lymphocytes at a reasonable price.