Measurement of IL-8

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Pharmacology
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Quality of Results

Ease-of-Optimization

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Company:

Bio-Ocean

Product Name:

ELISA IL-8 kit

Catalog Number:

EK108

My project was related to sepsis and some possible inhibitors of sepsis related receptors. We wanted to check how my inhibitors are effective, so as an important proinflammatory cytokine/chemokine, IL-8, was a key player there. For this issue we used the kit from Biocompare, and we got promising results.

Experimental Design and Results Summary

Application

Measurement of IL-8

Starting Material

Monocyte immune cells

Protocol Overview

Incubation overnight with capture antibody. Then block the plate and add your samples. Afterwards, add the detection antibody and the enzyme. Then add the substrate and the stop solution. Analyze with the plate reader.

Tips

Work in the dark with the enzyme.

Results Summary

Promissing results for our inhibitors.

Additional Notes

None

Image Gallery

Summary

The Good

Saves time and it is easy to work with it.

The Bad

Small volume

The Bottom Line

I would suggest use of this kit. You can work properly, fast and easy.

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