eBioscience
Anti-Mouse F4/80 Antigen APC-eFluor® 780
47-4801-80
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Peritoneal macrophages were identified by CD11b and F4/80.
Flow Cytometry
Naive Peritoneal Cells
1:200 F4/80-APC/eF780 and 1:400 CD11b-BV605 on ice for 30 minutes
1:100 FcBlock - 5 minutes on ice
NA
LSRII Flow Cytometer
Macrophages were easily identified as F480hi, CD11b+.
None
Bright staining
F4/80-APC/eF780 works well for identification of macrophages in the peritoneal cavity.