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Brilliant Violet 605™ anti-mouse CD274 (B7-H1, PD-L1) Antibody
124321
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This antibody was used in an experiment to identify the expression pattern of PD-L1 on splenic DC and macrophages.
Flow Cytometry
Splenocytes
+4c, 30 mins
Anti-CD16/32
NA
LSR II
We observed that PD-L1 is expressed by CD11c-hi DC. It was a decent separation between positive and background. Care should be taken in voltage adjustment and compensation between BV605 and BV510 (if you're also using this fluorochrome).
None
Decent antibody to detect PD-L1 expression
Potentially high compensation needed between BV605 and BV510.
Definitely worth a try.