Human CD3 (Brilliant Violet 510™)

Immunology
Universitätsklinikum Hamburg-Eppendorf
BTA

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

Brilliant Violet 510™ anti-human CD3

Catalog Number:

317332

We are using this antibody on full cordblood in a 15 colour panel. This antibody is good to distinguish the T cell population and gate the different cell subsets from there.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Whole cord blood

Primary Incubation

0.1µL CD3 in 50µL FACS buffer added to 50µL blood for 30min at RT

Blocking Agent

NA

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

BD LSR Fortessa

Results Summary

Good detection of CD3+ T cells and very clear separation between positive and negative populations even in low concentrations.

Additional Notes

None

Related Categories

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Summary

The Good

The antibody gives very good results in low concentrations.

The Bad

None

The Bottom Line

This antibody is good for multi-colour panel.

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