Rat Leptin Estimation ELISA Kit

National Institute of Nutrition
Biochemistry
Senior research fellow

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Company:

R&D Systems

Product Name:

Mouse/Rat Leptin Quantikine ELISA Kit, 2 Plate

Catalog Number:

MOB00

Leptin is a hormone produced by adipose cells that regulate energy balance by stopping hunger. Using the principle of sandwich ELISA, this kit measures the leptin levels via polyclonal antibodies which are pre coated in plate that bind to leptin in the samples. 

Experimental Design and Results Summary

Application

This kit is using for estimating the leptin levels in rat serum and plasma.

Starting Material

Obese rat plasma samples

Protocol Overview

Collected rat plasma using EDTA tubes and Centrifuged for 20 minutes at 2000 x g within 30 minutes of collection. Assayed immediately according to the protocol in the kit

Tips

Always use fresh samples. Avoid repeated freeze thaw cycles.

Results Summary

Observed higher leptin levels in obese rats vs controls.

Additional Notes

None

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Summary

The Good

Supplied almost all necessary reagents in the kit.

The Bad

A little expensive

The Bottom Line

It is really good for leptin levels estimation if cost is manageable.

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