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Brilliant Violet 421™ anti-mouse/human KLRG1 (MAFA) antibody
138413
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Browse Similar Products: KLRG1 Flow Cytometry Antibodies
KLRG-1 is expressed on memory T cells and NK cells and has been reported to exhibit inhibitory functions in these cell types. In this pilot experiment, I looked at the expression of KLRG-1 on CD4 T cells.
Flow Cytometry
Splenocytes
+4C, 30 mins
Anti-CD16/32
NA
LSR II
Cells were first stained with cell surface markers (anti-CD3 and CD4). This was followed by staining with Fixable blue, a viability dye. Cells were then fixed/permeabilized with the Foxp3 Fix/Perm buffer from eBioscience. Staining with anti-KLRG1 was performed in 100ul of 1xWash buffer @1:100 or 1:400 dilution. This antibody gave an excellent separation between positive cells and background.
None
Great antibody
Highly recommended.