Decent Vinvulin Antibody

March 10, 2016

Research
University of North Carolina at Chapel Hill
Laboratory Technician

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technology

Product Name:

Vinculin (E1E9V) XP® Rabbit mAb (HRP Conjugate)

Catalog Number:

18799

Our lab uses Vinculin as a loading control in a number of our western blots, and we usually buy our antibodies from Cell Signaling because of their great reputation.

Experimental Design and Results Summary

Applications

Western Blot

Sample

786-0 Cell Lysates

Primary Incubation

1:1000 Dilution Overnight in 5% BSA in 1X TBS-T at 4C

Blocking Agent

5% Milk in 1X TBS-T

Secondary Incubation

None needed - already HRP-conjugated

Tertiary Incubation

None

Detection

Autoradiography Film using ECL alone

Results Summary

This antibody works pretty well, but it's somewhat dirty as well. If you don't need a really crisp blot, it'll do the job for you. Still, it's somewhat disappointing that this antibody isn't of better quality.

Additional Notes

None

Related Categories

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Summary

The Good

Works pretty well

The Bad

Expensive

The Bottom Line

This may not be the best choice of Vinculin antibody suppliers. For the money, you could probably do better purchasing the antibody somewhere else. Still, it does the job if you don't need a really good antibody.

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