Cell Signaling Technology
p53 (1C12) Mouse mAb
2524
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Browse Similar Products: p53 Western Blot Antibodies
Like many other cancer labs, our lab works with p53. We recently needed to detect p53 protein levels in 786-0 cell lysates, and gave this antibody a try first on a recommendation from several other labs.
Western Blot
786-0 Cell Lysates
1:1000 Dilution Overnight in 5% BSA in 1X TBS-T at 4C
5% Milk in 1X TBS-T
1:5000 dilution of HRP-conjugated Mouse secondary in 5% Milk in TBS-T for 1 hour at RT
NA
ECL --> Autoradiography Film via HRP-Conjugate Secondary Antibody
Great quality antibody. We were able to visualize protein levels quite well using this antibody at a number of different protein levels. It didn't require any additional optimization and saved us a great deal of time by not requiring any.
None
High quality, Clean
Expensive
Cell Signaling is known for the quality of their antibodies and customer service. This p53 antibody is well-worth the price and carries that same reputation. I highly recommend it.