Cell Signaling Technology
Phospho-Src Family (Tyr416) Antibody
2101
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Our lab works to elucidate the impact of kinases in cancer progression, and our research often includes Western Blots in order to determine kinase protein and activity levels in various cancer cell lines. In particular, we were working to detect endogenous levels of the phosphorylated Src family of kinases in 786-0 cell lysates. We chose this CST antibody because of their reputable customer service and well-developed antibodies.
Western Blot
786-0 Cell Lysates
1:1000 Dilution in 5% BSA in 1X TBS-T at 4C Overnight
5% Milk in 1X TBS-T
1:5000 dilution of HRP-conjugated Rabbit secondary in 5% Milk in TBS-T for 1 hour at RT
None
ECL/FEMTO --> Autoradiography Film via HRP-Conjugated Rabbit Secondary Antibody
Using this antibody, we were able to detect accurately the levels of phosphorylated Src family kinases in 786-0 cell lysates. This allowed us to draw further conclusions about the influence of the Src family of kinases upon cancer progression in several types of cancers. Using autoradiography film and a low-strength mixture of ECL, there was very little background and a strong signal. Around 15-30 seconds of exposure time was sufficient to produce adequate bands on film.
Re-use the antibody to get a cleaner result
Clean, Works Great
Expensive
This antibody is worth it. If you need to detect the Tyr416 residue reliably, I highly recommend that you take advantage of this antibody.